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Overview of iris microvessel architecture. Vessels are ordered radially
around the pupil (bottom right corner). |
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Rhodamine labelled leukocytes (white dots). Red blood cells can be
visualized as well. (pupil lower right) |
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Overview magnification (200x) at baseline (top) and 4 hrs after intravitreal
endotoxin injection. Infiltrating cells (arrows) can be seen and counted easily. |
 |
Rhodamine labelled leukocytes increasingly roll, stick, and infiltrate
over time. High magnification (500x) of the same iris vessels at baseline (top),
2 hrs (middle), and 4 hrs (bottom) after intravitreal endotoxin injection into normal
BALB/c mice. |
 |
No change in leukocyte migration in control animals. High magnification
(500x) of the same iris vessels at baseline (top), 2 hrs (middle), and 4 hrs (bottom)
after intravitreal vehicle injection into normal BALB/c mice. |
 |
This is an iris wholemount image of a normal BALB/c mouse at baseline.
The pupil is located in the lower lefthand corner. Vessels are stained red and leukocytes
are brown. |
 |
This is an iris wholemount image of a normal BALB/c mouse 4 hours
after induction of inflammation by intravitreal LPS injection. Vessels are stained
red and leukocytes brown. |
 |
Comparison of whole mount iris immunohistology (left panel) and in
vivo observations (right panel) 6 hrs after LPS-injection. The top photos represent
an IL-8 knockout mouse. The bottom photos represent a control mouse.
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